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cluster:73 [2019/08/27 13:31]
hmeij07 [Kallisto & Trinity]
cluster:73 [2019/09/23 12:05]
hmeij07 [R & R/parallel]
Line 262: Line 262:
  
 ====== Kallisto & Trinity & FastQC====== ====== Kallisto & Trinity & FastQC======
 +
 +**FastQC** A quality control tool for high throughput sequence data.
 +
 +  * ''java -version'' latest JDK installed, in default PATH ''which java''
 +  * location: /share/apps/FastQC/0.11.8
 +  * https://www.bioinformatics.babraham.ac.uk/projects/fastqc/
 +
  
 **kallisto** is a program for quantifying abundances of transcripts from bulk and single-cell RNA-Seq data, or more generally of target sequences using high-throughput sequencing reads. **kallisto** is a program for quantifying abundances of transcripts from bulk and single-cell RNA-Seq data, or more generally of target sequences using high-throughput sequencing reads.
Line 379: Line 386:
  
  
-**FastQC** A quality control tool for high throughput sequence data. 
  
-  * ''java -version'' latest JDK installed, in default PATH ''which java'' 
-  * location: /share/apps/FastQC/0.11.8 
-  * https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ 
  
 ====== Sequencing Tools ====== ====== Sequencing Tools ======
Line 1822: Line 1825:
 ====== R & R/parallel ====== ====== R & R/parallel ======
  
 +  * R 3.6.1 (running against "packages", had to comment out libcurl version check)
 +  * New java jdk-12.0.2 (by default in your path)
 +  * packages lme4 and tidyverse installed (dependencies=TRUE)
 +  * https://www.r-bloggers.com/what-are-the-best-machine-learning-packages-in-r/
 +  * packages: mice, rpart, party, caret(does not exist), randomforest, nnet, e1071, kernLab
 +
 +<code>
 +
 +  export PATH=/share/apps/CENTOS6/R/packages/bin:$PATH
 +  export LD_LIBRARY_PATH=/share/apps/CENTOS6/R/packages/lib:$LD_LIBRARY_PATH
 +  export PATH=/share/apps/CENTOS6/R/3.6.1/bin:$PATH
 +  export LD_LIBRARY_PATH=/share/apps/CENTOS6/R/3.6.1/lib64:$LD_LIBRARY_PATH
 +  export PATH=/share/apps/CENTOS6/gcc/4.8.4/bin:$PATH
 +  export LD_LIBRARY_PATH=/share/apps/CENTOS6/gcc/4.8.4/lib64:/share/apps/CENTOS6/gcc/4.8.4/lib:$LD_LIBRARY_PATH
 +
 +  #export PATH=/share/apps/java/jdk-12.0.2/bin:$PATH
 +  #export LD_LIBRARY_PATH=/share/apps/java/jdk-12.0.2/lib:$LD_LIBRARY_PATH
 +
 +</code>
  
   * R 3.5.1 (new libcurl package)   * R 3.5.1 (new libcurl package)
-  * Uses same old java home 1.7.0_21, should remember to update+  * Uses same old java home 1.7.0_21 (jdk or jre?), should remember to update
   * package lme4 installed   * package lme4 installed
  
Line 1833: Line 1855:
 export PATH=/home/apps/CENTOS6/R/3.5.1/bin:$PATH export PATH=/home/apps/CENTOS6/R/3.5.1/bin:$PATH
 export LD_LIBRARY_PATH=/home/apps/CENTOS6/R/3.5.1/lib64:$LD_LIBRARY_PATH export LD_LIBRARY_PATH=/home/apps/CENTOS6/R/3.5.1/lib64:$LD_LIBRARY_PATH
 +
 +export PATH=/share/apps/java/jdk1.7.0_21/bin:$PATH
 +export LD_LIBRARY_PATH=/share/apps/java/jdk1.7.0_21/lib:$LD_LIBRARY_PATH
  
 </code> </code>
cluster/73.txt ยท Last modified: 2024/05/15 12:58 by hmeij07