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--- cluster:73 [2021/07/13 15:55]
hmeij07 [Miniconda3]
+++ cluster:73 [2021/07/15 14:00]
hmeij07 [Sequencing Tools #3]
@@ Line 44: / Line 44: @@
 which conda python pip mpirun gcc cmake
 python --version
+</code>
+** Guppy Server **
+  * https://github.com/nanoporetech/megalodon, find community page
+  * standalone build
+  * Local accelerated basecalling for Nanopore data
+  * v5.0.11
+<code>
+export PATH=/share/apps/CENTOS7/guppy/5.0.11/ont-guppy-cpu/bin:$PATH
+export LD_LIBRARY_PATH=/share/apps/CENTOS7/guppy/5.0.11/ont-guppy-cpu/lib:$LD_LIBRARY_PATH
+ldd `which guppy_basecall_server`
+# cpu; note as user start server on scratch server greentail52
+guppy_basecall_server --config dna_r9.4.1_450bps_fast.cfg -p 5555 -l /tmp/guppy &
+# check if running, use ''kill'' command to terminate
+lsof -i:5555
+COMMAND     PID      USER   FD   TYPE    DEVICE SIZE/OFF NODE NAME
+guppy_bas 80002 bchernoff   12u  IPv4 959121280      0t0  TCP *:personal-agent (LISTEN)
+# nodes can reach this server at this IP or start their own on localhost:port
+.168.102.251 greentail52-eth0
+.10.102.251   greentail52-eth1 (preferred)
+</code>
+** Guppy Client **
+  * https://github.com/nanoporetech/pyguppyclient
+  * miniconda3 python3.9
+  * Python client library for Guppy.
+  * Full Python client library for communicating with guppy_basecall_server.
+  * v0.1.0
+<code>
+pip install cython PyUnicode timer
+pip install pyguppyclient
+Successfully installed flatbuffers-1.11 ont-fast5-api-3.3.0 pyguppyclient-0.0.6 pyzmq-17.1.2
+note: from pyguppyclient import GuppyBasecallerClient, yield_reads
+success, but do not know how to execute the example
 </code>

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