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cluster:73 [2023/07/04 12:22]
hmeij07 [Sequencing Tools #3]
cluster:73 [2023/07/12 14:15]
hmeij07 [Trimmomatic]
Line 26: Line 26:
  
  
-====== Trimmomatic ======+===== Masurca ===== 
 + 
 + 
 +    module: masurca/4.1.0 
 +    https://github.com/alekseyzimin/masurca/releases 
 +    supports openmp (maybe) 
 +    queues mwgpu, exx96 (centos 7) 
 + 
 + 
 +<code> 
 + 
 +export PATH=/share/apps/CENTOS7/masurca/4.1.0/bin:$PATH 
 +export LD_LIBRARY_PATH=/share/apps/CENTOS7/masurca/4.1.0/lib:$LD_LIBRARY_PATH 
 + 
 +</code> 
 + 
 +===== Trimmomatic =====
  
   * http://www.usadellab.org/cms/?page=trimmomatic   * http://www.usadellab.org/cms/?page=trimmomatic
Line 346: Line 362:
 ** Flye ** ** Flye **
  
-Somewhat annoying, no miniconda3 package, so local install, use minconda3 environment as listed top of this section (python 3.9, gcc 9.2). Local building (without installation) on centos7 greentail52.+Somewhat annoying, no miniconda3 package, (python 3.9, gcc 9.2). Local building (without installation) on centos7 greentail52.
  
 +  * setup env as for all packages in #3, see top of section
   * v2.9.2 (June 2023)   * v2.9.2 (June 2023)
   * python bin/flye   * python bin/flye
-    * chnage the PATHS accordingly+    * change the PATHS accordingly
  
 <code> <code>
Line 1091: Line 1108:
 pcangsd --version pcangsd --version
 pcangsd 1.10 pcangsd 1.10
 +
 +</code>
 +
 +  * **angsd**, requires miniconda3, same environment as pcangsd
 +  * http://www.popgen.dk/angsd/index.php/RealSFS
 +  * add location of angsd to PATH env 
 +
 +<code>
 +
 +$ pwd
 +/share/apps/CENTOS7/angsd/0.940/angsd
 +
 +$ ls -l angsd
 +-rwxr-xr-x 1 hmeij its 5517248 Jul  6 14:11 angsd
 +
 +$ ./angsd 
 + -> angsd version: 0.940-dirty (htslib: 1.16) build(Jul  6 2023 14:11:30)
  
 </code> </code>
cluster/73.txt · Last modified: 2024/05/15 12:58 by hmeij07